Antoine Griezmann scored the 57th minute spot kick.
In a World Cup first, referee Andres Cunha did not initially award a penalty after Greizmann was brought down by Joshua Risdon.
He was alerted by the VAR to a possible penalty and went over to the pitch side monitor.
On review, he decided it was a spot kick.
The VAR system is making its first appearance at a World Cup.
Four VAR officials, sitting in a room at the International Broadcasting Centre on the outskirts of Moscow are assisting match officials at the tournament.